金川多肋牦牛Hoxa6和Hoxa10基因甲基化与mRNA差异表达研究

doi:10.11843/j.issn.0366-6964.2015.09.007

Abstract

Abstract:

The transcription level and methylation in promoter regions of Hoxa6 and Hoxa10 genes in multi-costa properties and common yak were investigated to explore the mechanism of transcriptional regulation of Hox gene in the process of multi-costa properties.The mRNA expression leves of Hoxa6 and Hoxa10 genes in multi-costa properties and common yak were detected by real-time fluorescence quantitative PCR(qRT-PCR)，and the methylation of Hoxa6 and Hoxa10 genes promoter regions were analyzed by bisulfite-sequencing PCR(BSP)，which included modifying，cloning and sequencing the promoter regions.The results showed that the expression level of Hoxa6 gene was significantly higher in multi-costa properties yak than that in common yak(P<0.05)，while the expression level of Hoxa10 gene was significantly lower in multi-costa properties yak(P<0.05).The methylation status of CpG2 island in the Hoxa6 promoter region was significantly different between multi-costa properties and common yak，especially on No.3，4，8，20 and 21 CpG sites.However，the methylation status of CpG1 island in the Hoxa10 promoter region was significantly higher in multi-costa properties yak than that in common yak(P<0.05)，and there was almost no methylation on No.9 and 12 CpG sites in common yak.All above results suggested that the high DNA hypermethylation of Hoxa10 gene in multi-costa properties yak inhibited the expression of Hoxa10 gene in a certain degree，and the low DNA hypomethylation of Hoxa6 gene enhanced the expression of Hoxa6 gene.It was concluded that methylation in Hox promoter regions may have some influence on transcription regulation of Hox genes，and there may be some correlation with the formation of multi-costa properties.

CLC Number:

XIONG Xian-rong，ZHANG Yan，LAN Dao-liang，LI Jian,ZI Xiang-dong，LI Shan-rong，YANG Jian-mei. Differences of Hoxa6 and Hoxa10 Methylation Status and mRNA Expression in Multi-costa Properties of Jinchuan Yak[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(9): 1532-1539.

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Differences of Hoxa6 and Hoxa10 Methylation Status and mRNA Expression in Multi-costa Properties of Jinchuan Yak

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